Cation Transport Coupled to ATP Hydrolysis By the (Na, K)-ATPase : an integrated, animated model


Autoria(s): Leone Francisco A.; Furriel Rosa P. M.; McNamara John C.; Horisberger Jean D.; Borin Ivana A.
Data(s)

2010

Resumo

An Adobe (R) animation is presented for use in undergraduate Biochemistry courses, illustrating the mechanism of Na+ and K+ translocation coupled to ATP hydrolysis by the (Na, K)-ATPase, a P-2c-type ATPase, or ATP-powered ion pump that actively translocates cations across plasma membranes. The enzyme is also known as an E-1/E-2-ATPase as it undergoes conformational changes between the E-1 and E-2 forms during the pumping cycle, altering the affinity and accessibility of the transmembrane ion-binding sites. The animation is based on Horisberger's scheme that incorporates the most recent significant findings to have improved our understanding of the (Na, K)-ATPase structure function relationship. The movements of the various domains within the (Na, K)-ATPase alpha-subunit illustrate the conformational changes that occur during Na+ and K+ translocation across the membrane and emphasize involvement of the actuator, nucleotide, and phosphorylation domains, that is, the "core engine" of the pump, with respect to ATP binding, cation transport, and ADP and P-i release.

Identificador

http://serval.unil.ch/?id=serval:BIB_DF29094AB7AB

isbn:1470-8175

isiid:000281266700014

doi:10.1002/bmb.20404

Idioma(s)

en

Fonte

Biochemistry and Molecular Biology Education, vol. 38, no. 4, pp. 276-279

Palavras-Chave #enzymes and catalysis; original models for teaching and learning; transport through membranes; using multimedia in the classroom; SODIUM-POTASSIUM PUMP; P-TYPE ATPASES; CALCIUM-PUMP; SARCOPLASMIC-RETICULUM; CRYSTAL-STRUCTURE; BINDING-SITES; NA,K-ATPASE; MECHANISM; H,K-ATPASE
Tipo

info:eu-repo/semantics/article

article