Cation Transport Coupled to ATP Hydrolysis By the (Na, K)-ATPase : an integrated, animated model
Data(s) |
2010
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Resumo |
An Adobe (R) animation is presented for use in undergraduate Biochemistry courses, illustrating the mechanism of Na+ and K+ translocation coupled to ATP hydrolysis by the (Na, K)-ATPase, a P-2c-type ATPase, or ATP-powered ion pump that actively translocates cations across plasma membranes. The enzyme is also known as an E-1/E-2-ATPase as it undergoes conformational changes between the E-1 and E-2 forms during the pumping cycle, altering the affinity and accessibility of the transmembrane ion-binding sites. The animation is based on Horisberger's scheme that incorporates the most recent significant findings to have improved our understanding of the (Na, K)-ATPase structure function relationship. The movements of the various domains within the (Na, K)-ATPase alpha-subunit illustrate the conformational changes that occur during Na+ and K+ translocation across the membrane and emphasize involvement of the actuator, nucleotide, and phosphorylation domains, that is, the "core engine" of the pump, with respect to ATP binding, cation transport, and ADP and P-i release. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_DF29094AB7AB isbn:1470-8175 isiid:000281266700014 doi:10.1002/bmb.20404 |
Idioma(s) |
en |
Fonte |
Biochemistry and Molecular Biology Education, vol. 38, no. 4, pp. 276-279 |
Palavras-Chave | #enzymes and catalysis; original models for teaching and learning; transport through membranes; using multimedia in the classroom; SODIUM-POTASSIUM PUMP; P-TYPE ATPASES; CALCIUM-PUMP; SARCOPLASMIC-RETICULUM; CRYSTAL-STRUCTURE; BINDING-SITES; NA,K-ATPASE; MECHANISM; H,K-ATPASE |
Tipo |
info:eu-repo/semantics/article article |