A high-efficiency HeLa cell nuclear transcription extract.


Autoria(s): Shapiro D.J.; Sharp P.A.; Wahli W.W.; Keller M.J.
Data(s)

01/02/1988

Resumo

A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard HeLa cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5-4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template.

Identificador

http://serval.unil.ch/?id=serval:BIB_D7A76779C6CA

isbn:1044-5498 and 0198-0238[print], 0198-0238[linking]

pmid:3349904

doi:10.1089/dna.1988.7.47

isiid:A1988M104700006

Idioma(s)

en

Fonte

DNA, vol. 7, no. 1, pp. 47-55

Palavras-Chave #Adenoviridae/genetics; Animals; Cell Nucleus/metabolism; Cell-Free System; Hela Cells/metabolism; Humans; Promoter Regions, Genetic; Transcription, Genetic; Vitellogenins/genetics; Xenopus/genetics
Tipo

info:eu-repo/semantics/article

article