A high-efficiency HeLa cell nuclear transcription extract.
Data(s) |
01/02/1988
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Resumo |
A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard HeLa cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5-4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_D7A76779C6CA isbn:1044-5498 and 0198-0238[print], 0198-0238[linking] pmid:3349904 doi:10.1089/dna.1988.7.47 isiid:A1988M104700006 |
Idioma(s) |
en |
Fonte |
DNA, vol. 7, no. 1, pp. 47-55 |
Palavras-Chave | #Adenoviridae/genetics; Animals; Cell Nucleus/metabolism; Cell-Free System; Hela Cells/metabolism; Humans; Promoter Regions, Genetic; Transcription, Genetic; Vitellogenins/genetics; Xenopus/genetics |
Tipo |
info:eu-repo/semantics/article article |