Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.


Autoria(s): Corthésy B.; Claret F.X.; Wahli W.
Data(s)

01/10/1990

Resumo

Female-specific expression of the Xenopus laevis vitellogenin gene was reconstituted in vitro by addition of recombinant vaccinia-virus-produced estrogen receptor to nuclear extracts from male livers, in which this gene is silent. Transcription enhancement was at least 30 times and was selectively restricted to vitellogenin templates containing the estrogen-responsive unit. Thus, in male hepatocytes, estrogen receptor is the limiting regulatory factor that in the female liver controls efficient and accurate sex-specific expression of the vitellogenin gene. Furthermore, the Xenopus liver factor B, which is essential in addition to the estrogen receptor for the activation of this gene, was successfully replaced in the Xenopus extract by purified human nuclear factor I, identifying factor B of Xenopus as a functional homolog of this well-characterized human transcription factor.

Identificador

http://serval.unil.ch/?id=serval:BIB_C20236845AE4

isbn:0027-8424[print], 0027-8424[linking]

pmid:2236004

doi:10.1073/pnas.87.20.7878

isiid:A1990ED61200018

Idioma(s)

en

Fonte

Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 20, pp. 7878-7882

Palavras-Chave #Animals; Base Sequence; CCAAT-Enhancer-Binding Proteins; Cell Nucleus/metabolism; Chromosome Deletion; DNA-Binding Proteins/metabolism; Female; Humans; Male; Molecular Sequence Data; Mutagenesis, Site-Directed; NFI Transcription Factors; Nuclear Proteins; Oligonucleotide Probes; Promoter Regions, Genetic; Receptors, Estrogen/physiology; Restriction Mapping; Sex Factors; Templates, Genetic; Transcription Factors; Transcription, Genetic; Vitellogenins/genetics; Xenopus laevis; Y-Box-Binding Protein 1
Tipo

info:eu-repo/semantics/article

article