Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.


Autoria(s): Goy G.; Croxatto A.; Posfay-Barbe K.M.; Gervaix A.; Greub G.
Data(s)

2009

Resumo

Waddlia chondrophila is considered as an emerging human pathogen likely involved in miscarriage and lower respiratory tract infections. Given the low sensitivity of cell culture to recover such an obligate intracellular bacteria, molecular-based diagnostic approaches are warranted. We thus developed a real-time PCR that amplifies Waddlia chondrophila DNA. Specific primers and probe were selected to target the 16S rRNA gene. The PCR specifically amplified W. chondrophila but did not amplify other related-bacteria such as Parachlamydia acanthamoebae, Simkania negevensis and Chlamydia pneumoniae. The PCR exhibited a good intra-run and inter-run reproducibility and a sensitivity of less than ten copies of the positive control. This real-time PCR was then applied to 32 nasopharyngeal aspirates taken from children with bronchiolitis not due to respiratory syncytial virus (RSV). Three samples revealed to be Waddlia positive, suggesting a possible role of this Chlamydia-related bacteria in this setting.

Identificador

http://serval.unil.ch/?id=serval:BIB_BD348AF19844

isbn:1435-4373 (Electronic)

pmid:19727868

doi:10.1007/s10096-009-0804-7

isiid:000271531200012

Idioma(s)

en

Fonte

European Journal of Clinical Microbiology and Infectious Diseases, vol. 28, no. 12, pp. 1483-1486

Palavras-Chave #Bacteriological Techniques/methods; Child; Chlamydiales/genetics; Chlamydiales/isolation & purification; DNA Primers/genetics; DNA, Bacterial/genetics; DNA, Ribosomal/genetics; Female; Gram-Negative Bacterial Infections/diagnosis; Gram-Negative Bacterial Infections/microbiology; Humans; Infant; Infant, Newborn; Nasopharynx/microbiology; Polymerase Chain Reaction/methods; RNA, Ribosomal, 16S/genetics; Reproducibility of Results; Sensitivity and Specificity
Tipo

info:eu-repo/semantics/article

article