Strigolactone Promotes Degradation of DWARF14, an α/β Hydrolase Essential for Strigolactone Signaling in Arabidopsis.


Autoria(s): Chevalier F.; Nieminen K.; Sánchez-Ferrero J.C.; Rodríguez M.L.; Chagoyen M.; Hardtke C.S.; Cubas P.
Data(s)

2014

Resumo

Strigolactones (SLs) are phytohormones that play a central role in regulating shoot branching. SL perception and signaling involves the F-box protein MAX2 and the hydrolase DWARF14 (D14), proposed to act as an SL receptor. We used strong loss-of-function alleles of the Arabidopsis thaliana D14 gene to characterize D14 function from early axillary bud development through to lateral shoot outgrowth and demonstrated a role of this gene in the control of flowering time. Our data show that D14 distribution in vivo overlaps with that reported for MAX2 at both the tissue and subcellular levels, allowing physical interactions between these proteins. Our grafting studies indicate that neither D14 mRNA nor the protein move over a long range upwards in the plant. Like MAX2, D14 is required locally in the aerial part of the plant to suppress shoot branching. We also identified a mechanism of SL-induced, MAX2-dependent proteasome-mediated degradation of D14. This negative feedback loop would cause a substantial drop in SL perception, which would effectively limit SL signaling duration and intensity.

Identificador

http://serval.unil.ch/?id=serval:BIB_B57A9C89882A

isbn:1532-298X (Electronic)

pmid:24610723

doi:10.1105/tpc.114.122903

isiid:000334932700025

Idioma(s)

en

Fonte

Plant Cell, vol. 26, no. 3, pp. 1134-1150

Tipo

info:eu-repo/semantics/article

article