Superantigen-induced immune stimulation amplifies mouse mammary tumor virus infection and allows virus transmission.


Autoria(s): Held W.; Waanders G.A.; Shakhov A.N.; Scarpellino L.; Acha-Orbea H.; MacDonald H.R.
Data(s)

1993

Resumo

Endogenous and infectious mouse mammary tumor viruses (MMTVs) encode in their 3' long terminal repeat a protein that exerts superantigen activity; that is, it is able to interact with T cells via the variable domain of the T cell receptor (TCR) beta chain. We show here that transmission of an infectious MMTV is prevented when superantigen-reactive cells are absent through either clonal deletion due to the expression of an endogenous MTV with identical superantigen specificity or exclusion due to expression of a transgenic TCR beta chain that does not interact with the viral superantigen. A strict requirement for superantigen-reactive T cells is also seen for a local immune response following MMTV infection. This immune response locally amplifies the number of MMTV-infected B cells, most likely owing to their clonal expansion. Collectively, our data indicate that a superantigen-induced immune response is critical for the MMTV life cycle.

Identificador

http://serval.unil.ch/?id=serval:BIB_958C4C50E223

isbn:0092-8674

pmid:8394220

doi:10.1016/0092-8674(93)80054-I

isiid:A1993LT73900014

Idioma(s)

en

Fonte

Cell, vol. 74, no. 3, pp. 529-540

Palavras-Chave #Animals; Antigens, Viral/biosynthesis; Antigens, Viral/immunology; B-Lymphocytes/immunology; Base Sequence; DNA, Viral/genetics; DNA, Viral/isolation & purification; Flow Cytometry; Lymph Nodes/microbiology; Mammary Neoplasms, Experimental/immunology; Mammary Neoplasms, Experimental/microbiology; Mammary Tumor Virus, Mouse/genetics; Mammary Tumor Virus, Mouse/immunology; Mice; Mice, Inbred BALB C; Mice, Transgenic; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction; Receptors, Antigen, T-Cell, alpha-beta/metabolism; Repetitive Sequences, Nucleic Acid; T-Lymphocytes/immunology
Tipo

info:eu-repo/semantics/article

article