Identification of HIV integration sites in infected host genomic DNA.


Autoria(s): Ciuffi A.; Barr S.D.
Data(s)

2011

Resumo

The integration of the Human Immunodeficiency Virus (HIV) genetic information into the host genome is fundamental for its replication and long-term persistence in the host. Isolating and characterizing the integration sites can be useful for obtaining data such as identifying the specific genomic location of integration or understanding the forces dictating HIV integration site selection. The methods outlined in this article describe a highly efficient and precise technique for identifying HIV integration sites in the host genome on a small scale using molecular cloning techniques and standard sequencing or on a massive scale using 454 pyrosequencing.

Identificador

http://serval.unil.ch/?id=serval:BIB_8EED600CB038

isbn:1095-9130 (Electronic)

pmid:20385239

doi:10.1016/j.ymeth.2010.04.004

isiid:000286708000007

http://my.unil.ch/serval/document/BIB_8EED600CB038.pdf

http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_8EED600CB0385

Idioma(s)

en

Direitos

info:eu-repo/semantics/openAccess

Fonte

Methods, vol. 53, no. 1, pp. 39-46

Tipo

info:eu-repo/semantics/article

article