TCRgamma silencing during alphabeta T cell development depends upon pre-TCR-induced proliferation.


Autoria(s): Ferrero I.; Mancini S.J.; Grosjean F.; Wilson A.; Otten L.; MacDonald H.R.
Data(s)

2006

Resumo

During thymus development, immature T cells become committed to two distinct lineages based upon expression of alphabeta or gammadelta TCR. In the alphabeta lineage, developing thymocytes progressively extinguish transcription of the TCRgamma genes by a poorly understood process known as gamma silencing. We show that alphabeta lineage thymocytes in mice lacking a functional pre-TCR undergo limited proliferation and fail to silence TCRgamma genes during development. Stimulation of pre-TCR-deficient immature thymocytes with anti-CD3 Abs does not directly down-regulate TCRgamma transcription but restores TCRgamma silencing following proliferation. Collectively our data reveal an important role for pre-TCR induced proliferation in activating the TCRgamma silencer in alphabeta lineage thymocytes, a process that may reinforce alphabeta or gammadelta lineage commitment.

Identificador

http://serval.unil.ch/?id=serval:BIB_8E100BA9B454

isbn:0022-1767

pmid:17056529

isiid:000241477400033

Idioma(s)

en

Fonte

Journal of immunology, vol. 177, no. 9, pp. 6038-6043

Palavras-Chave #Animals; Antibodies/pharmacology; Antigens, CD3/immunology; Cell Lineage/genetics; Cell Proliferation; Gene Silencing; Mice; Receptors, Antigen, T-Cell, alpha-beta/agonists; Receptors, Antigen, T-Cell, alpha-beta/analysis; Receptors, Antigen, T-Cell, gamma-delta/agonists; Receptors, Antigen, T-Cell, gamma-delta/genetics; Silencer Elements, Transcriptional/genetics; T-Lymphocyte Subsets/drug effects; T-Lymphocyte Subsets/immunology; Thymus Gland/cytology; Thymus Gland/growth & development
Tipo

info:eu-repo/semantics/article

article