Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.


Autoria(s): Rappaz B.; Cano E.; Colomb T.; Kühn J.; Depeursinge C.; Simanis V.; Magistretti P.J.; Marquet P.
Data(s)

2009

Resumo

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

Identificador

http://serval.unil.ch/?id=serval:BIB_8258ED09645A

isbn:1083-3668

pmid:19566341

doi:10.1117/1.3147385

isiid:000268536300057

Idioma(s)

en

Fonte

Journal of biomedical optics, vol. 14, no. 3, pp. 034049

Tipo

info:eu-repo/semantics/article

article