Analysis of the enantiomers of citalopram and its demethylated metabolites using chiral liquid chromatography.
Data(s) |
1998
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Resumo |
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal standard, were recovered from plasma by liquid-liquid extraction. The limits of quantification were found to be 5 ng/ml for each enantiomer of citalopram and demethylcitalopram, and 7.5 ng/ml for each enantiomer of didemethylcitalopram. Inter- and intra-day coefficients of variation varied from 2.4% to 8.6% for S- and R-citalopram, from 2.9% to 7.4% for S- and R-demethylcitalopram, and from 5.6% to 12.4% for S- and R- didemethylcitalopram. No interference was observed from endogenous compounds following the extraction of plasma samples from 10 different patients treated with citalopram. This method allows accurate quantification for each enantiomer and is, therefore, well suited for pharmacokinetic and drug interaction investigations. The presented method replaces a previously described highly sensitive and selective high-performance liquid chromatography procedure using an acetylated 3-cyclobond column which, because of manufactural problems, is no longer usable for the separation of the enantiomers of citalopram and its demethylated metabolites. |
Identificador |
https://serval.unil.ch/?id=serval:BIB_78BA074FA16B isbn:1387-2273 (Print) pmid:9869386 isiid:000077327500029 doi:10.1016/S0378-4347(98)00384-3 |
Idioma(s) |
en |
Fonte |
Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 719, no. 1-2, pp. 234-238 |
Palavras-Chave | #Antidepressive Agents, Second-Generation/blood; Antidepressive Agents, Second-Generation/pharmacokinetics; Calibration; Chromatography, High Pressure Liquid/methods; Citalopram/blood; Citalopram/pharmacokinetics; Humans; Reproducibility of Results; Serotonin Uptake Inhibitors/blood; Serotonin Uptake Inhibitors/pharmacokinetics; Spectrometry, Fluorescence; Stereoisomerism |
Tipo |
info:eu-repo/semantics/article article |