Methods for integration site distribution analyses in animal cell genomes.


Autoria(s): Ciuffi, A.; Ronen, K.; Brady, T.; Malani, N.; Wang, G.; Berry, C.C.; Bushman, F.D.
Data(s)

01/04/2009

Resumo

The question of where retroviral DNA becomes integrated in chromosomes is important for understanding (i) the mechanisms of viral growth, (ii) devising new anti-retroviral therapy, (iii) understanding how genomes evolve, and (iv) developing safer methods for gene therapy. With the completion of genome sequences for many organisms, it has become possible to study integration targeting by cloning and sequencing large numbers of host-virus DNA junctions, then mapping the host DNA segments back onto the genomic sequence. This allows statistical analysis of the distribution of integration sites relative to the myriad types of genomic features that are also being mapped onto the sequence scaffold. Here we present methods for recovering and analyzing integration site sequences.

Identificador

https://serval.unil.ch/notice/serval:BIB_78620AD3F739

info:pmid:19038346

pmid:19038346

doi:10.1016/j.ymeth.2008.10.028

isiid:000265812600007

Idioma(s)

eng

Fonte

Methods474261-268

Palavras-Chave #retrovirus, integration site
Tipo

info:eu-repo/semantics/article

article