Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
| Data(s) |
2010
|
|---|---|
| Resumo |
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction. |
| Identificador |
http://serval.unil.ch/?id=serval:BIB_7561C44C2B0E isbn:1098-5530[electronic], 0021-9193[linking] pmid:20543073 doi:10.1128/JB.00492-10 isiid:000280406300020 |
| Idioma(s) |
en |
| Fonte |
Journal of Bacteriology, vol. 192, no. 16, pp. 4251-4255 |
| Palavras-Chave | #Aminobutyric Acids/metabolism; Anti-Bacterial Agents/biosynthesis; Antimetabolites/metabolism; Biosynthetic Pathways/genetics; Gene Order; Genes, Bacterial; Multigene Family; Mutagenesis, Site-Directed; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/metabolism |
| Tipo |
info:eu-repo/semantics/article article |
