Expression of Fas (APO-1/CD95) and Fas ligand (FasL) in human neuroblastoma.


Autoria(s): Gross N.; Balmas K.; Beretta Brognara C.; Tschopp J.
Data(s)

2001

Resumo

BACKGROUND AND PROCEDURE: To determine the possible role of Fas/FasL system in the particularly heterogeneous behaviour of neuroblastoma (NB), we have measured the functional expression of Fas and its ligand, FasL, in primary neuroblastoma samples and cell lines by immunohistochemistry and flow cytometry. RESULTS: Our results reveal that while Fas expression is associated with low stage and more mature tumors, heterogeneous FasL expression was mostly detected in high stage tumors, with our apparent correlation to MYCN amplification. Flow cytometric analysis of cell lines demonstrated a high expression of Fas in epithelial-type, HLA class I positive cell lines, which was lost upon activation with phorbol esters. In contrast, Fas ligand was detected in only a small subset of cell lines. CONCLUSIONS: In some cell lines, cytotoxic assays revealed the ability of NB-associated Fas receptor to transduce an apoptotic signal upon triggering. The pattern of functional Fas/FasL expression in tumours and cell lines suggests that this system may be involved in the evasion of highly malignant neuroblastoma cells to host immune response.

Identificador

http://serval.unil.ch/?id=serval:BIB_66245D9F0135

isbn:0098-1532

pmid:11464860

doi:10.1002/1096-911X(20010101)36:1<111::AID-MPO1026>3.0.CO;2-3

isiid:000166167300026

Idioma(s)

en

Fonte

Medical and pediatric oncology, vol. 36, no. 1, pp. 111-4

Palavras-Chave #Antigens, CD95; Apoptosis; Fas Ligand Protein; Flow Cytometry; Ganglioneuroma; Gene Amplification; Gene Expression Regulation, Neoplastic; Genes, myc; Humans; Jurkat Cells; Membrane Glycoproteins; Neoplasm Proteins; Neoplasm Staging; Neuroblastoma; Tumor Cells, Cultured
Tipo

info:eu-repo/semantics/article

article