Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector


Autoria(s): Rodríguez Pizà, Ignasi; Barragan, Montserrat; González, Federico; Izpisúa Belmonte, J. C.; Batlle Morera, Laura; Vassena, Rita; Montserrat, Nuria; Tiscornia, Gustavo
Data(s)

02/07/2013

Resumo

Induced pluripotent stem (iPS) cells have generated keen interestdue to their potential use in regenerative medicine. They havebeen obtained from various cell types of both mice and humans byexogenous delivery of different combinations of Oct4, Sox2, Klf4,c-Myc, Nanog, and Lin28. The delivery of these transcription factorshas mostly entailed the use of integrating viral vectors (retrovirusesor lentiviruses), carrying the risk of both insertional mutagenesisand oncogenesis due to misexpression of these exogenousfactors. Therefore, obtaining iPS cells that do not carry integratedtransgene sequences is an important prerequisite for their eventualtherapeutic use. Here we report the generation of iPS cell linesfrom mouse embryonic fibroblasts with no evidence of integrationof the reprogramming vector in their genome, achieved by nucleofectionof a polycistronic construct coexpressing Oct4, Sox2, Klf4,and c-Myc

Identificador

http://hdl.handle.net/10230/12427

Idioma(s)

eng

Publicador

National Academy of Sciences

Direitos

(c) National Academy of Sciences

info:eu-repo/semantics/openAccess

Palavras-Chave #Medicina regenerativa #Cél·lules mare embrionàries
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion