Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.
Autoria(s):
Morata, Pilar; Queipo-Ortuño, María Isabel; de Dios Colmenero, Juan de Dios
Data(s)
02/03/2016
02/03/2016
01/09/1998
Resumo
We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.
Journal Article; Research Support, Non-U.S. Gov't;
This research was supported by FIS grant 97/0713 and funds from PAI 54/97 Consejeria de Salud, Junta de Andalucia, Spain.
Identificador
Morata P, Queipo-Ortuño MI, Colmenero J de D. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998; 36(9):2443-6
