Neutrophil defensins: their possible role in allergic asthma.

BACKGROUND Neutrophil defensins, originally identified as broad-spectrum antimicrobial peptides, have been implicated in the regulation of inflammatory and immunological processes. OBJECTIVES To investigate whether the in vitro challenge of neutrophils from patients with bronchial asthma with allergens stimulated the release of alpha-defensins and whether levels released were dependent on lung infections. METHOD The neutrophils were cultivated with different agonists and the concentration of alpha-defensin in cell-free supernatant was measured with enzyme-linked immunosorbent assay (ELISA). RESULTS Neutrophils from allergic patients released alpha-defensins via an allergen-dependent mechanism. Our results indicate that the in vitro activation of neutrophils is highly allergen-specific. In this context, allergens other than those which produced clinical symptoms did not elicit alpha-defensin release, and allergens had no effect on neutrophils from healthy donors. However, neutrophils from both allergic patients and healthy controls were able to release alpha-defensins upon treatment with PMA. In the allergen-stimulated neutrophils, cells from asthmatic patients stimulated with a sensitizing allergen showed a significantly higher production of alpha-defensin under respiratory tract infection than cells from the same patients without such an infection. CONCLUSION Neutrophils from allergic patients release alpha-defensins via an allergen-dependent mechanism.

Journal Article; Research Support, Non-U.S. Gov't;

This work was supported by grants from the Junta de Andalucía (Ayudas Grupos de Investigación), the Asociación Sanitaria Virgen Macarena, Sevilla, and Fundación Alergol, Sevilla. Spain. A.V. was supported by fellowships from the Ministerio de Ciencia y Tecnología and Fundación Alergol, Spain. I.V. was supported by a fellowship from the Asociación Sanitaria Virgen Macarena and J.M. is a participant of the Programa de Intensificación de la Actividad Investigadora of the Spanish National Health System