UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.
| Data(s) |
26/10/2015
26/10/2015
01/10/2013
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| Resumo |
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria. |
| Identificador |
Cejudo-García MA, Muñoz-Davila MJ, Martínez-Lirola MJ. UV-fixed-thick-blotch preparation improves sensitivity of auramine staining. J. Clin. Microbiol.. 2013 ; 51(10):3400-2 1098-660X (Online) 0095-1137 (Print) PMC3811656 http://hdl.handle.net/10668/2036 23863571 10.1128/JCM.00974-13 |
| Idioma(s) |
es |
| Publicador |
American Society for Microbiology |
| Relação |
Journal of Clinical Microbiology http://jcm.asm.org/content/51/10/3400.abstract |
| Direitos |
Acceso abierto |
| Palavras-Chave | #Tuberculosis #Mycobacterium #Técnicas bacteriológicas #Benzofenoneido #Esputo #Manejo de especímenes #Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis #Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Rods::Gram-Positive Asporogenous Rods::Gram-Positive Asporogenous Rods, Regular::Mycobacteriaceae::Mycobacterium #Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques #Medical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amines::Aniline Compounds::Benzophenoneidum #Medical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Sputum #Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Specimen Handling |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/published Artículo |
