Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis.
| Data(s) |
02/12/2015
02/12/2015
2015
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|---|---|
| Resumo |
Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P < 0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P < 0.0001) and was related to the severity of the lesions (Spearman's rho = 0.775, P = 0.0001). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair. Journal Article; This investigation was partially supported by Research Group #CTS-138 (Junta de Andalucía, Spain) |
| Identificador |
O'Valle F, Peregrina M, Crespo-Lora V, Galindo-Moreno P, Roman M, Padial-Molina M, et al. Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis. Biomed Res Int. 2015:681456 2314-6141 (Online) PMC4433648 http://hdl.handle.net/10668/2097 26064941 10.1155/2015/681456 |
| Idioma(s) |
en |
| Publicador |
Hindawi Publishing Corporation |
| Relação |
BioMed Research International http://www.hindawi.com/journals/bmri/2015/681456/abs/ |
| Direitos |
Acceso abierto |
| Palavras-Chave | #Modelos teóricos #Artritis experimental #Colágeno tipo II #Ratones #Células madre adultas #Inmunización #Inmunoglobulina g #Articulaciones #Animales #Masculino #Factor de necrosis tumoral alfa #Receptores del factor de necrosis tumoral #Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical #Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Animal::Disease Models, Animal::Arthritis, Experimental #Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen #Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice #Medical Subject Headings::Anatomy::Cells::Stem Cells::Adult Stem Cells #Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type II #Medical Subject Headings::Health Care::Health Care Facilities, Manpower, and Services::Health Services::Preventive Health Services::Primary Prevention::Immunization #Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G #Medical Subject Headings::Anatomy::Musculoskeletal System::Skeleton::Joints #Medical Subject Headings::Organisms::Eukaryota::Animals #Medical Subject Headings::Check Tags::Male #Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha #Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Tumor Necrosis Factor |
| Tipo |
info:eu-repo/semantics/article info:eu-repo/semantics/published Artículo |
