DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis


Autoria(s): Forster,Karine M; Hartwig,Daiane D; Oliveira,Thaís L; Bacelo,Kátia L; Schuch,Rodrigo; Amaral,Marta G; Dellagostin,Odir A
Data(s)

01/12/2015

Resumo

Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.

Formato

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Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800989

Idioma(s)

en

Publicador

Instituto Oswaldo Cruz, Ministério da Saúde

Fonte

Memórias do Instituto Oswaldo Cruz v.110 n.8 2015

Palavras-Chave #leptospirosis #Leptospira #LigBrep #subunit vaccine #DNA vaccine #prime-boost
Tipo

journal article