High-frequency linkage of co-expressing T-DNA in transgenic Arabidopsis thaliana transformed by vacuum-infiltration of Agrobacterium tumefaciens
Data(s) |
2000
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Resumo |
The efficiency of co-expression and linkage of distinct T-DNAs present in separate Agrobacterium tumefaciens was analysed in Arabidopsis thaliana transformed by the vacuum infiltration method. Co-expression was monitored by the synthesis of three bacterial proteins involved in the production of polyhydroxybutyrate (PHB) in the plastids. Out of 80 kanamycin-resistant transgenic plants analysed, 13 plants were co-transformed with the two distinct T-DNAs and produced PHB. Of those, 7 lines had a kanamycin-resistance segregation ratio consistent with the presence of a single functional insert. Genetic linkage between the distinct T-DNAs was demonstrated for all 13 PHB-producing lines, while physical linkage between the distinct T-DNAs was shown for 12 out of 13 lines. T-DNAs were frequently linked in an inverted orientation about the left borders. Transformation of A. thaliana by the co-infiltration of two A. tumefaciens containing distinct T-DNAs is, thus, an efficient approach for the integration and expression of several transgenes at a single locus. This approach will facilitate the creation and study of novel metabolic pathways requiring the expression of numerous transgenes. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_4483BE973EF3 isbn:0040-5752 doi:10.1007/s001220050063 isiid:000086145800020 |
Idioma(s) |
en |
Fonte |
Theoretical and Applied Genetics, vol. 100, no. 3-4, pp. 487-493 |
Palavras-Chave | #Arabidopsis thaliana; T-DNA; co-transformation; linkage; Agrobacterium tumefaciens |
Tipo |
info:eu-repo/semantics/article article |