High-frequency linkage of co-expressing T-DNA in transgenic Arabidopsis thaliana transformed by vacuum-infiltration of Agrobacterium tumefaciens


Autoria(s): Poirier Y.; Ventre G.; Nawrath C.
Data(s)

2000

Resumo

The efficiency of co-expression and linkage of distinct T-DNAs present in separate Agrobacterium tumefaciens was analysed in Arabidopsis thaliana transformed by the vacuum infiltration method. Co-expression was monitored by the synthesis of three bacterial proteins involved in the production of polyhydroxybutyrate (PHB) in the plastids. Out of 80 kanamycin-resistant transgenic plants analysed, 13 plants were co-transformed with the two distinct T-DNAs and produced PHB. Of those, 7 lines had a kanamycin-resistance segregation ratio consistent with the presence of a single functional insert. Genetic linkage between the distinct T-DNAs was demonstrated for all 13 PHB-producing lines, while physical linkage between the distinct T-DNAs was shown for 12 out of 13 lines. T-DNAs were frequently linked in an inverted orientation about the left borders. Transformation of A. thaliana by the co-infiltration of two A. tumefaciens containing distinct T-DNAs is, thus, an efficient approach for the integration and expression of several transgenes at a single locus. This approach will facilitate the creation and study of novel metabolic pathways requiring the expression of numerous transgenes.

Identificador

http://serval.unil.ch/?id=serval:BIB_4483BE973EF3

isbn:0040-5752

doi:10.1007/s001220050063

isiid:000086145800020

Idioma(s)

en

Fonte

Theoretical and Applied Genetics, vol. 100, no. 3-4, pp. 487-493

Palavras-Chave #Arabidopsis thaliana; T-DNA; co-transformation; linkage; Agrobacterium tumefaciens
Tipo

info:eu-repo/semantics/article

article