Obesity-associated insulin resistance is correlated to adipose tissue vascular endothelial growth factors and metalloproteinase levels.


Autoria(s): Tinahones, Francisco José; Coín-Aragüez, Leticia; Mayas, María Dolores; García-Fuentes, Eduardo; Hurtado-Del-Pozo, Carmen; Vendrell, Joan; Cardona, Fernando; Calvo, Rosa-María; Obregón, María-Jesus; El Bekay, Rajaa
Data(s)

19/03/2013

19/03/2013

01/04/2012

Resumo

BACKGROUND The expansion of adipose tissue is linked to the development of its vasculature, which appears to have the potential to regulate the onset of obesity. However, at present, there are no studies highlighting the relationship between human adipose tissue angiogenesis and obesity-associated insulin resistance (IR). RESULTS Our aim was to analyze and compare angiogenic factor expression levels in both subcutaneous (SC) and omentum (OM) adipose tissues from morbidly obese patients (n = 26) with low (OB/L-IR) (healthy obese) and high (OB/H-IR) degrees of IR, and lean controls (n = 17). Another objective was to examine angiogenic factor correlations with obesity and IR.Here we found that VEGF-A was the isoform with higher expression in both OM and SC adipose tissues, and was up-regulated 3-fold, together with MMP9 in OB/L-IR as compared to leans. This up-regulation decreased by 23% in OB/-H-IR compared to OB/L-IR. On the contrary, VEGF-B, VEGF-C and VEGF-D, together with MMP15 was down-regulated in both OB/H-IR and OB/L-IR compared to lean patients. Moreover, MMP9 correlated positively and VEGF-C, VEGF-D and MMP15 correlated negatively with HOMA-IR, in both SC and OM. CONCLUSION We hereby propose that the alteration in MMP15, VEGF-B, VEGF-C and VEGF-D gene expression may be caused by one of the relevant adipose tissue processes related to the development of IR, and the up-regulation of VEGF-A in adipose tissue could have a relationship with the prevention of this pathology.

Journal Article; Research Support, Non-U.S. Gov't;

This work was supported in part by grants from the Andalusian Health Service [SAS PI-0251], from Fondos de Investigación Sanitaria PS09/00997, Instituto Carlos III, from Incentivos a Proyectos de Excelencia p08-CTS-04369. Consejería de Innovación, Junta de Andalucía, and Rajaa El Bekay is recipient of a post-doctoral grant "Miguel Servet" (FIS-2007) (CP07/00288) from the Spanish Ministry of Health; and part of this project was supported by a grant from the Spanish Ministry of Health (FIS) (PI070953), and from Plan Nacional (MEC) SAF2009-09364 and S2010-BMD-2423 from Comunidad de Madrid. CIBEROBN is an initiative of ISCIII (Instituto de Salud Carlos III), Spain.

Identificador

Tinahones FJ, Coín-Aragüez L, Mayas MD, García-Fuentes E, Hurtado-del-Pozo C, Vendrell J, et al. Obesity-associated insulin resistance is correlated to adipose tissue vascular endothelial growth factors and metalloproteinase levels. BMC Physiol. 2012; 12:4

1472-6793 (Online)

PMC3382430

http://hdl.handle.net/10668/860

22471305

10.1186/1472-6793-12-4

Idioma(s)

en

Publicador

BioMed Central

Relação

BMC physiology

http://www.biomedcentral.com/1472-6793/12/4

Direitos

Acceso abierto

Palavras-Chave #Inductores de la Angiogénesis #Marcadores Biológicos #Expresión Génica #Humanos #Resistencia a la Insulina #Metaloproteasas #Neovascularización Fisiológica #Obesidad #Obesidad Mórbida #Epiplón #Grasa Subcutánea #Factor A de Crecimiento Endotelial Vascular #Tejido Adiposo #Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Growth Substances::Angiogenesis Modulating Agents::Angiogenesis Inducing Agents #Medical Subject Headings::Chemicals and Drugs::Biological Factors::Biological Markers #Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression #Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans #Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Insulin Resistance #Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Metalloproteases #Medical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Cardiovascular Physiological Phenomena::Cardiovascular Physiological Processes::Neovascularization, Physiologic #Medical Subject Headings::Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Overweight::Obesity #Medical Subject Headings::Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity::Obesity, Morbid #Medical Subject Headings::Anatomy::Body Regions::Torso::Abdomen::Abdominal Cavity::Peritoneum::Omentum #Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous Fat #Medical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Angiogenic Proteins::Vascular Endothelial Growth Factors::Vascular Endothelial Growth Factor A #Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Tipo

info:eu-repo/semantics/article

info:eu-repo/semantics/published

Artículo