The redox potential interferes with the expression of laminin binding molecules in Bacteroides fragilis


Autoria(s): Ferreira,Eliane de Oliveira; Yates,Edwin Alexander; Goldner,Morris; Vommaro,Rossiane Cláudia; Silva Filho,Fernando Costa e; Petrópolis,Débora Barreiros; Domingues,Regina MC Pilotto
Data(s)

01/11/2008

Resumo

The Bacteroides fragilis ATCC strain was grown in a synthetic media with contrasting redox potential (Eh) levels [reduced (-60 mV) or oxidised (+100mV)] and their adhesion capacity to extracellular matrix components was evaluated. The strain was capable of adhering to laminin, fibronectin, fibronectin + heparan sulphate and heparan sulphate. A stronger adherence to laminin after growing the strain under oxidising conditions was verified. Electron microscopy using ruthenium red showed a heterogeneous population under this condition. Dot-blotting analyses confirmed stronger laminin recognition by outer membrane proteins of cells cultured at a higher Eh. Using a laminin affinity column, several putative laminin binding proteins obtained from the cultures kept under oxidising (60 kDa, 36 kDa, 25 kDa and 15 kDa) and reducing (60 kDa) conditions could be detected. Our results show that the expression of B. fragilis surface components that recognise laminin are influenced by Eh variations.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000700010

Idioma(s)

en

Publicador

Instituto Oswaldo Cruz, Ministério da Saúde

Fonte

Memórias do Instituto Oswaldo Cruz v.103 n.7 2008

Palavras-Chave #Bacteroides fragilis #adhesion #redox potential #laminin binding proteins #outer membrane proteins
Tipo

journal article