An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7).


Autoria(s): Held W.; Shakhov A.N.; Waanders G.; Scarpellino L.; Luethy R.; Kraehenbuhl J.P.; MacDonald H.R.; Acha-Orbea H.
Data(s)

1992

Resumo

The classical minor lymphocyte stimulating (Mls) antigens, which induce a strong primary T cell response in vitro, are closely linked to endogenous copies of mouse mammary tumor viruses (MMTV). Expression of Mls genes leads to clonal deletion of T cell subsets expressing specific T cell receptor (TCR) V beta chains. We describe the isolation and characterization of a new exogenous (infectious) MMTV with biological properties similar to the Mls antigen Mls-1a. In vivo administration of either Mls-1a-expressing B cells or the infectious MMTV (SW) led to an increase of T cells expressing V beta 6 followed by their deletion. Surprisingly, different kinetics of deletion were observed with the exogenous virus depending upon the route of infection. Infection through the mucosa led to a slow deletion of V beta 6+ T cells, whereas deletion was rapid after subcutaneous infection. Sequence analysis of the open reading frames in the 3' long terminal repeat of both this exogenous MMTV (SW) and of Mtv-7 (which is closely linked to Mls-1a) revealed striking similarities, particularly in the COOH terminus, which has been implicated in TCR V beta recognition. The identification of an infectious MMTV with the properties of a strong Mls antigen provides a new, powerful tool to study immunity and tolerance in vivo.

Identificador

http://serval.unil.ch/?id=serval:BIB_34B1C10DA8A9

isbn:0022-1007 (Print)

pmid:1316932

doi:10.1084/jem.175.6.1623

isiid:A1992HV67600024

Idioma(s)

en

Fonte

Journal of Experimental Medicine, vol. 175, no. 6, pp. 1623-1633

Palavras-Chave #Amino Acid Sequence; Animals; Antibodies, Monoclonal; Base Sequence; Cloning, Molecular; Female; Genes, Viral; Lymph Nodes/immunology; Mammary Tumor Virus, Mouse/genetics; Mammary Tumor Virus, Mouse/isolation & purification; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred Strains; Milk/microbiology; Minor Lymphocyte Stimulatory Antigens/analysis; Minor Lymphocyte Stimulatory Antigens/genetics; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction/methods; Receptors, Antigen, T-Cell/genetics; Sequence Homology, Nucleic Acid; Species Specificity; T-Lymphocytes/immunology; Thymus Gland/immunology
Tipo

info:eu-repo/semantics/article

article