Establishing two-dimensional gels for the analysis of Leishmania proteomes.


Autoria(s): Acestor N.; Masina S.; Walker J.; Saravia N.G.; Fasel N.; Quadroni M.
Data(s)

2002

Resumo

Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.

Identificador

http://serval.unil.ch/?id=serval:BIB_33D358FBBC78

isbn:1615-9853 (Print)

pmid:12124933

doi:10.1002/1615-9861(200207)2:7<877::AID-PROT877>3.0.CO;2-D

isiid:000177056800010

Idioma(s)

en

Fonte

Proteomics, vol. 2, no. 7, pp. 877-879

Palavras-Chave #Animals; Electrophoresis, Gel, Two-Dimensional/methods; Leishmania/chemistry; Leishmania/genetics; Molecular Weight; Proteome/chemistry; Protozoan Proteins/analysis
Tipo

info:eu-repo/semantics/article

article