Establishing two-dimensional gels for the analysis of Leishmania proteomes.
Data(s) |
2002
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Resumo |
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_33D358FBBC78 isbn:1615-9853 (Print) pmid:12124933 doi:10.1002/1615-9861(200207)2:7<877::AID-PROT877>3.0.CO;2-D isiid:000177056800010 |
Idioma(s) |
en |
Fonte |
Proteomics, vol. 2, no. 7, pp. 877-879 |
Palavras-Chave | #Animals; Electrophoresis, Gel, Two-Dimensional/methods; Leishmania/chemistry; Leishmania/genetics; Molecular Weight; Proteome/chemistry; Protozoan Proteins/analysis |
Tipo |
info:eu-repo/semantics/article article |