Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing.


Autoria(s): Boccard J.; Badoud F.; Jan N.; Nicoli R.; Schweizer C.; Pralong F.; Veuthey J.L.; Baume N.; Rudaz S.; Saugy M.
Data(s)

2014

Resumo

AIM: Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse. RESULTS: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted. CONCLUSION: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.

Identificador

http://serval.unil.ch/?id=serval:BIB_2B44DB382204

isbn:1757-6199 (Electronic)

pmid:25411696

doi:10.4155/bio.14.200

isiid:000345341800003

Idioma(s)

en

Fonte

Bioanalysis, vol. 6, no. 19, pp. 2523-2536

Tipo

info:eu-repo/semantics/article

article