Isoelectric focusing of alpha-1 acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system.
| Data(s) |
1988
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|---|---|
| Resumo |
A method allowing a clear separation of the different variants of desialylated alpha 1-acid glycoprotein (orosomucoid) has been developed using isoelectric focusing in immobilized pH gradients, supplemented with 8 M urea and 2% v/v 2-mercaptoethanol. Immunoblotting with two antibody-steps afforded high sensitivity and permitted the detection of about 700 pg of alpha 1-acid glycoprotein in a 20 microL plasma sample diluted 1:28 672. A one year old bloodstrain, kept at room temperature, could easily be phenotyped. |
| Identificador |
https://serval.unil.ch/?id=serval:BIB_2A23F6FF4D1E isbn:0173-0835 (Print) pmid:3243228 doi:10.1002/elps.1150091005 isiid:A1988R055600082 |
| Idioma(s) |
en |
| Fonte |
Electrophoresis, vol. 9, no. 10, pp. 650-654 |
| Palavras-Chave | #Alkaline Phosphatase; Blood Stains; Genetic Variation; Humans; Hydrogen-Ion Concentration; Immunoblotting; Isoelectric Focusing/methods; Neuraminidase; Orosomucoid/genetics; Orosomucoid/isolation & purification; Phenotype; Urea |
| Tipo |
info:eu-repo/semantics/article article |
