Hydrophobic labeling of (Na+,K+)-ATPase: further evidence that the beta subunit is embedded in the membrane bilayer.
Data(s) |
1983
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Resumo |
O-Hexanoyl-3,5-diiodo-N-(4-azido-2-nitro-phenyl)tyramine has been used after photochemical conversion into the reactive nitrene to label (Na+,K+)-ATPase from Bufo marinus toad kidney. Immunochemical evidence indicates that the reagent labels both subunits of the enzyme in partially purified form as well as in microsomal membranes. These results support the view that the glycoprotein subunit, like the catalytic subunit, possesses hydrophobic domains by which it is integrated into the plasma membrane. |
Identificador |
http://serval.unil.ch/?id=serval:BIB_24EA03F7F256 isbn:0006-2960 pmid:6305414 doi:10.1021/bi00278a037 isiid:A1983QM57500037 |
Idioma(s) |
en |
Fonte |
Biochemistry, vol. 22, no. 9, pp. 2296-300 |
Palavras-Chave | #Animals; Bufo marinus; Indicators and Reagents; Kidney; Kinetics; Lipid Bilayers; Macromolecular Substances; Microsomes; Sodium-Potassium-Exchanging ATPase; Tyramine |
Tipo |
info:eu-repo/semantics/article article |