Survival of tubercle bacilli in heat-fixed and stained sputum smears
Data(s) |
01/02/2001
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Resumo |
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis. |
Formato |
text/html |
Identificador |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024 |
Idioma(s) |
en |
Publicador |
Instituto Oswaldo Cruz, Ministério da Saúde |
Fonte |
Memórias do Instituto Oswaldo Cruz v.96 n.2 2001 |
Palavras-Chave | #tubercle bacilli #slide culture #sputum smear |
Tipo |
journal article |