Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species


Autoria(s): Rosa,EAR; Rosa,RT; Pereira,CV; Boriollo,MFG; Höfling,JF
Data(s)

01/12/2000

Resumo

Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000600009

Idioma(s)

en

Publicador

Instituto Oswaldo Cruz, Ministério da Saúde

Fonte

Memórias do Instituto Oswaldo Cruz v.95 n.6 2000

Palavras-Chave #polyacrylamide gel electrophoresis #multilocus enzyme electrophoresis #Candida #numerical analysis
Tipo

journal article