Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
| Data(s) |
01/04/2000
|
|---|---|
| Resumo |
Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification. |
| Formato |
text/html |
| Identificador |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015 |
| Idioma(s) |
en |
| Publicador |
Instituto Oswaldo Cruz, Ministério da Saúde |
| Fonte |
Memórias do Instituto Oswaldo Cruz v.95 n.2 2000 |
| Palavras-Chave | #bovine herpesvirus-1 #cosolvents #polymerase chain reaction |
| Tipo |
journal article |
