Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.


Autoria(s): Orioli A.; Pascali C.; Quartararo J.; Diebel K.W.; Praz V.; Romascano D.; Percudani R.; van Dyk L.F.; Hernandez N.; Teichmann M.; Dieci G.
Data(s)

2011

Resumo

Human RNA polymerase (Pol) III-transcribed genes are thought to share a simple termination signal constituted by four or more consecutive thymidine residues in the coding DNA strand, just downstream of the RNA 3'-end sequence. We found that a large set of human tRNA genes (tDNAs) do not display any T(≥4) stretch within 50 bp of 3'-flanking region. In vitro analysis of tDNAs with a distanced T(≥4) revealed the existence of non-canonical terminators resembling degenerate T(≥5) elements, which ensure significant termination but at the same time allow for the production of Pol III read-through pre-tRNAs with unusually long 3' trailers. A panel of such non-canonical signals was found to direct transcription termination of unusual Pol III-synthesized viral pre-miRNA transcripts in gammaherpesvirus 68-infected cells. Genome-wide location analysis revealed that human Pol III tends to trespass into the 3'-flanking regions of tDNAs, as expected from extensive terminator read-through. The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3'-trailer sequences with the potential to contribute novel functional ncRNAs.

Identificador

https://serval.unil.ch/?id=serval:BIB_17DFDA5C12C3

isbn:1362-4962 (Electronic)

pmid:21421562

doi:10.1093/nar/gkr074

isiid:000293020000022

http://my.unil.ch/serval/document/BIB_17DFDA5C12C3.pdf

http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_17DFDA5C12C31

Idioma(s)

en

Direitos

info:eu-repo/semantics/openAccess

Fonte

Nucleic Acids Research, vol. 39, no. 13, pp. 5499-5512

Palavras-Chave #3' Flanking Region; Animals; Cell Line; Hela Cells; Humans; Mice; RNA Polymerase III/metabolism; RNA, Transfer/genetics; Sequence Analysis, DNA; Terminator Regions, Genetic; Transcription, Genetic
Tipo

info:eu-repo/semantics/article

article