Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.
Data(s) |
2011
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Resumo |
Human RNA polymerase (Pol) III-transcribed genes are thought to share a simple termination signal constituted by four or more consecutive thymidine residues in the coding DNA strand, just downstream of the RNA 3'-end sequence. We found that a large set of human tRNA genes (tDNAs) do not display any T(≥4) stretch within 50 bp of 3'-flanking region. In vitro analysis of tDNAs with a distanced T(≥4) revealed the existence of non-canonical terminators resembling degenerate T(≥5) elements, which ensure significant termination but at the same time allow for the production of Pol III read-through pre-tRNAs with unusually long 3' trailers. A panel of such non-canonical signals was found to direct transcription termination of unusual Pol III-synthesized viral pre-miRNA transcripts in gammaherpesvirus 68-infected cells. Genome-wide location analysis revealed that human Pol III tends to trespass into the 3'-flanking regions of tDNAs, as expected from extensive terminator read-through. The widespread occurrence of partial termination suggests that the Pol III primary transcriptome in mammals is unexpectedly enriched in 3'-trailer sequences with the potential to contribute novel functional ncRNAs. |
Identificador |
https://serval.unil.ch/?id=serval:BIB_17DFDA5C12C3 isbn:1362-4962 (Electronic) pmid:21421562 doi:10.1093/nar/gkr074 isiid:000293020000022 http://my.unil.ch/serval/document/BIB_17DFDA5C12C3.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_17DFDA5C12C31 |
Idioma(s) |
en |
Direitos |
info:eu-repo/semantics/openAccess |
Fonte |
Nucleic Acids Research, vol. 39, no. 13, pp. 5499-5512 |
Palavras-Chave | #3' Flanking Region; Animals; Cell Line; Hela Cells; Humans; Mice; RNA Polymerase III/metabolism; RNA, Transfer/genetics; Sequence Analysis, DNA; Terminator Regions, Genetic; Transcription, Genetic |
Tipo |
info:eu-repo/semantics/article article |