Real-time PCR for type-specific identification of herpes simplex in clinical samples: evaluation of type-specific results in the context of CNS diseases.


Autoria(s): Meylan S.; Robert D.; Estrade C.; Grimbuehler V.; Péter O.; Meylan P.R.; Sahli R.
Data(s)

2008

Resumo

BACKGROUND: HSV-1 and HSV-2 cause CNS infections of dissimilar clinico-pathological characteristics with prognostic and therapeutic implications. OBJECTIVES: To validate a type-specific real-time PCR that uses MGB/LNA Taqman probes and to review the virologico-clinical data of 25 eligible patients with non-neonatal CNS infections. RESULTS: This real-time PCR was evaluated against conventional PCR (26 CSF and 20 quality controls), and LightCycler assay (51 mucocutaneous, 8 CSF and 32 quality controls) and culture/immunofluorescence (75 mucocutaneous) to assess typing with independent methods. Taqman real-time PCR detected 240 HSV genomes per ml CSF, a level appropriate for the management of patients, and provided unambiguous typing for the 104 positive (62 HSV-1 and 42 HSV-2) out the 160 independent clinical samples tested. HSV type diagnosed by Taqman real-time PCR predicted final diagnosis (meningitis versus encephalitis/meningoencephalitis, p<0.001) in 24/25 patients at time of presentation, in contrast to clinical evaluation. CONCLUSIONS: Our real-time PCR, as a sensitive and specific means for type-specific HSV diagnosis, provided rapid prognostic information for patient management.

Identificador

http://serval.unil.ch/?id=serval:BIB_107F5F6C2A56

isbn:1386-6532

pmid:18037340

doi:10.1016/j.jcv.2007.10.010

isiid:000253919100006

Idioma(s)

en

Fonte

Journal of Clinical Virology, vol. 41, no. 2, pp. 87-91

Palavras-Chave #Base Sequence; DNA Probes; Encephalitis, Viral/diagnosis; Encephalitis, Viral/virology; Herpes Simplex/diagnosis; Herpes Simplex/virology; Herpesvirus 1, Human/classification; Herpesvirus 1, Human/genetics; Herpesvirus 2, Human/classification; Herpesvirus 2, Human/genetics; Humans; Meningitis, Viral/diagnosis; Meningitis, Viral/virology; Molecular Sequence Data; Polymerase Chain Reaction/methods; Species Specificity; Viral Envelope Proteins/chemistry; Viral Envelope Proteins/genetics
Tipo

info:eu-repo/semantics/article

article