Lack of directed V alpha 14-J alpha 281 rearrangements in NK1+ T cells.


Autoria(s): Shimamura M.; Ohteki T.; Beutner U.; MacDonald H.R.
Data(s)

1997

Resumo

NK1.1+ T cells are an unusual subset of TCR alpha beta cells distinguished by their highly restricted V beta repertoire and predominant usage of an invariant V alpha 14-J alpha 281 chain. To assess whether a directed rearrangement mechanism could be responsible for this invariant alpha chain, we have analyzed V alpha 14 rearrangements by polymerase chain reaction and Southern blot in a panel of cloned T-T hybrids derived from thymic NK1.1+ T cells. As expected a high proportion (17/20) of the hybrids had rearranged V alpha 14 to J alpha 281. However, V alpha 14-J alpha 281 rearrangements always occurred on only one chromosome and were accompanied by other V alpha-J alpha rearrangements (not involving V alpha 14) on the homologous chromosome. These data argue that rigorous ligand selection rather than directed rearrangement is responsible for the high frequency of V alpha 14-J alpha 281 rearrangements in NK1.1+ T cells.

Identificador

http://serval.unil.ch/?id=serval:BIB_1017

isbn:0014-2980

pmid:9209513

doi:10.1002/eji.1830270638

isiid:A1997XD60100037

Idioma(s)

en

Fonte

European Journal of Immunology, vol. 27, no. 6, pp. 1576-1579

Palavras-Chave #Animals; Antigens/genetics; Antigens, Differentiation, B-Lymphocyte/chemistry; Antigens, Differentiation, B-Lymphocyte/genetics; Antigens, Ly; Antigens, Surface; Chromosomes/chemistry; Gene Rearrangement, T-Lymphocyte; Histocompatibility Antigens Class II/chemistry; Histocompatibility Antigens Class II/genetics; Lectins, C-Type; Mice; Mice, Inbred AKR; Mice, Inbred C57BL; NK Cell Lectin-Like Receptor Subfamily B; Proteins/genetics; Receptors, Antigen, T-Cell, alpha-beta/genetics; T-Lymphocyte Subsets/metabolism
Tipo

info:eu-repo/semantics/article

article