Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens


Autoria(s): Caterino-de-Araujo,Adele
Data(s)

01/06/1992

Resumo

The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200012

Idioma(s)

en

Publicador

Instituto Oswaldo Cruz, Ministério da Saúde

Fonte

Memórias do Instituto Oswaldo Cruz v.87 n.2 1992

Palavras-Chave #human immunodeficiency virus type 1 (HIV-1) #in vitro secretion of HIV-1-specific antibody #diagnosis of pediatric HIV-1 infection
Tipo

journal article