Development of nuclear DNA probes for the typing of Trypanososma cruzi


Autoria(s): Takeuchi,A. Mayumi; Traub-Cseko,Yara M.
Data(s)

01/09/1991

Resumo

We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005

Idioma(s)

en

Publicador

Instituto Oswaldo Cruz, Ministério da Saúde

Fonte

Memórias do Instituto Oswaldo Cruz v.86 n.3 1991

Palavras-Chave #Trypanosoma cruzi #random probes #typing #strains #numerical taxonomy
Tipo

journal article