Small ubiquitin-like modifier conjugating enzyme with active site mutation acts as dominant negative inhibitor of SUMO conjugation in arabidopsis(F).


Autoria(s): Tomanov K.; Hardtke C.; Budhiraja R.; Hermkes R.; Coupland G.; Bachmair A.
Data(s)

2013

Resumo

Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.

Identificador

https://serval.unil.ch/?id=serval:BIB_07FE518361F4

isbn:1744-7909 (Electronic)

pmid:23206124

doi:10.1111/jipb.12016

isiid:000318600300010

Idioma(s)

en

Fonte

Journal of Integrative Plant Biology, vol. 55, no. 1, pp. 75-82

Palavras-Chave #Arabidopsis/enzymology; Arabidopsis/metabolism; Blotting, Western; Mutation; Small Ubiquitin-Related Modifier Proteins/antagonists & inhibitors; Small Ubiquitin-Related Modifier Proteins/metabolism; Sumoylation; Transcription, Genetic; Ubiquitin/metabolism
Tipo

info:eu-repo/semantics/article

article