Evaluation of antibacterial activity of caffeic acid encapsulated by -cyclodextrins


Autoria(s): Pinho, Eva; Soares, Graça; Henriques, Mariana
Data(s)

2015

Resumo

Context: Caffeic acid is described as antibacterial, but this bioactive molecule has some issues regarding solubility and stability to environmental stress. Thus, encapsulation devices are required. Objective: The aim of this work was to study the effect of the caffeic acid encapsulation by cyclodextrins on its antibacterial activity. Materials and methods: The interactions between the caffeic acid and three cyclodextrins (-cyclodextrin (CD), 2-hydroxypropyl--cyclodextrin (HPCD) and methyl--cyclodextrin were study. Results and discussion: The formation of an aqueous soluble inclusion complex was confirmed for CD and HPCD with a 1:1 stoichiometry. The CD/caffeic acid complex showed higher stability than HPCD/caffeic acid. Caffeic acid antibacterial activity was similar at pH 3 and pH 5 against the three bacteria (K. pneumoniae, S. epidermidis and S. aureus). Conclusions: The antibacterial activity of the inclusion complexes was described here for the first time and it was shown that the caffeic acid activity was remarkably enhanced by the cyclodextrins encapsulation.

The authors have declared no conflict of interests. The authors are grateful for the FCT Strategic Project PEst-OE/EQB/LA0023/ 2013 and the Project ‘‘BioHealth – Biotechnology and Bioengineering approaches to improve health quality,’’ Ref. NORTE-07–0124-FEDER-000027, co-funded by the ‘‘Programa Operacional Regional do Norte’’ (ON.2 – O Novo Norte), QREN, FEDER. The authors also acknowledge the project ‘‘Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB’’, Ref. FCOMP-01– 0124-FEDER-027462. This work is, also, funded by FEDER funds through the Operational Programme for Competitiveness Factors - COMPETE and National Funds through FCT Foundation for Science and Technology under the project PEstC/CTM/UI0264/2011. Additionally, the authors would like to thank the FCT for the grant for E. Pinho (SFRH/BD/62665/2009).

Identificador

Pinho, Eva; Soares, Graça; Henriques, Mariana, Evaluation of antibacterial activity of caffeic acid encapsulated by -cyclodextrins. Journal of Microencapsulation: Micro and Nano Carriers, 32(8), 804-810, 2015

0265-2048

http://hdl.handle.net/1822/38525

1464-5246

10.3109/02652048.2015.1094531

Idioma(s)

eng

Publicador

Taylor & Francis

Relação

info:eu-repo/grantAgreement/FCT/3599-PPCDT/132966/PT

PEstC/CTM/UI0264/2011

SFRH/BD/62665/2009

http://www.tandfonline.com/action/journalInformation?journalCode=imnc20

Direitos

info:eu-repo/semantics/openAccess

Palavras-Chave #Staphylococcus aureus #inclusion complex #phenolic acid #MIC #wound infection
Tipo

info:eu-repo/semantics/article