In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity


Autoria(s): Santos,Lilian da Silva; Torres,Rosália Morais; Machado-de-Assis,Girley Francisco; Bahia,Maria Terezinha; Martins,Helen Rodrigues; Teixeira-Carvalho,Andréa; Coelho-dos-Reis,Jordana Grazziela Alves; Albajar-Viñas,Pedro; Martins-Filho,Olindo Assis; Lana,Marta de
Data(s)

01/02/2012

Resumo

INTRODUCTION: The goal was to develop an in-house serological method with high specificity and sensitivity for diagnosis and monitoring of Chagas disease morbidity. METHODS: With this purpose, the reactivities of anti-T. cruzi IgG and subclasses were tested in successive serum dilutions of patients from Berilo municipality, Jequitinhonha Valley, Minas Gerais, Brazil. The performance of the in-house ELISA was also evaluated in samples from other relevant infectious diseases, including HIV, hepatitis C (HCV), syphilis (SYP), visceral leishmaniasis (VL), and American tegumentary leishmaniasis (ATL), and noninfected controls (NI). Further analysis was performed to evaluate the applicability of this in-house methodology for monitoring Chagas disease morbidity into three groups of patients: indeterminate (IND), cardiac (CARD), and digestive/mixed (DIG/Mix), based on their clinical status. RESULTS: The analysis of total IgG reactivity at serum dilution 1:40 was an excellent approach to Chagas disease diagnosis (100% sensitivity and specificity). The analysis of IgG subclasses showed cross-reactivity, mainly with NI, VL, and ATL, at all selected serum dilutions. Based on the data analysis, the IND group displayed higher IgG3 levels and the DIG/Mix group presented higher levels of total IgG as compared with the IND and CARD groups. CONCLUSIONS: These findings demonstrated that methodology presents promising applicability in the analysis of anti-T. cruzi IgG reactivity for the differential diagnosis and evaluation of Chagas disease morbidity.

Formato

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Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100008

Idioma(s)

en

Publicador

Sociedade Brasileira de Medicina Tropical - SBMT

Fonte

Revista da Sociedade Brasileira de Medicina Tropical v.45 n.1 2012

Palavras-Chave #In-house ELISA #IgG subclasses #Differential Diagnosis #Chagas Disease Morbidity
Tipo

journal article