Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin®)


Autoria(s): Cardoso,Sandra Regina Afonso; Silva,João Carlos França da; Costa,Roberto Teodoro da; Mayrink,Wilson; Melo,Maria Norma; Michalick,Marilene Suzan Marques; Liu,Ibrahim Afrânio Willi; Fujiwara,Ricardo Toshio; Nascimento,Evaldo
Data(s)

01/04/2003

Resumo

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822003000200001

Idioma(s)

en

Publicador

Sociedade Brasileira de Medicina Tropical - SBMT

Fonte

Revista da Sociedade Brasileira de Medicina Tropical v.36 n.2 2003

Palavras-Chave #Tegumentary leishmaniasis #immunogenic proteins #Leishvacin
Tipo

journal article