Crystallographic studies on membrane and cytoplasmic enzymes

Dissertation presented to obtain the Ph.D degree in Biochemistry, Structural Biochemistry

Haloacid dehalogenase (HAD) superfamily (CL0137), includes a diverse range of enzymes that use an aspartate carboxylate as nucleophile (Aravind et al., 1998; Koonin and Tatusov, 1994). There are ~79000 sequences classified into this superfamily in Pfam database and even more sequences, 148000, classified as HAD-like in the UniProt database (as of 19th February 2013).(...)