hCES2: studies on the role of glycosylation in activity


Autoria(s): Alves, Márcia Maria da Silva
Contribuinte(s)

Simplício, Ana

Data(s)

19/12/2013

19/12/2013

2013

Resumo

Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Human carboxylesterase 2 (hCES2) is becoming more relevant due to its role in the hydrolysis of several drugs, pro-drugs and xenobiotics. However, its three dimensional structuture is yet to be resolved, and many of its properties are still unclear. In this work, the impact of glycosylation in the enzymatic activity of an hCES2 variant, hCES2-10xHis, was evaluated. To achieve this objective, different methods were used, such as enzymatic deglycosylation and glycosylation inhibition through specific inhibitors or plasmid mutation. It was concluded that glycosylation is necessary during hCES2 synthesis to maintain protein activity, but the presence of the glycans is not essential after the protein is completely matured.

Fundação para Ciência e Tecnologia - projecto (PTDC/EBB-BIO/111530/2009)

Identificador

http://hdl.handle.net/10362/10896

Idioma(s)

eng

Publicador

Faculdade de Ciências e Tecnologia

Direitos

openAccess

Palavras-Chave #Carboxylesterase #Glycosylation #Deglycosylation #PNGase F #Tunicamycin #Site directed mutagenesis
Tipo

masterThesis