Tnf-a production and apoptosis in hepatocytes after listeria monocytogenes and salmonella typhimurium invasion


Autoria(s): Santos,Sânia Alves Dos; Andrade Júnior,Dahir Ramos De; Andrade,Dahir Ramos De
Data(s)

01/04/2011

Resumo

Invasion of hepatocytes by Listeria monocytogenes (LM) and Salmonella Typhimurium (ST) can stimulate tumor necrosis factor alpha (TNF-α) release and induce apoptosis. In this study, we compared the behavior of hepatocytes invaded by three L. monocytogenes serotypes (LM-4a, LM-4b and LM-1/2a) and by ST to understand which bacterium is more effective in the infectious process. We quantified TNF-α release by ELISA, apoptosis rates by annexin V (early apoptosis) and TUNEL (late apoptosis) techniques. The cell morphology was studied too. TNF-α release rate was highest in ST-invaded hepatocytes. ST and LM-1/2a induced the highest apoptosis production rates evaluated by TUNEL. LM-4b produced the highest apoptosis rate measured by annexin. Invaded hepatocytes presented various morphological alterations. Overall, LM-4b and LM-1/2a proved to be the most efficient at cell invasion, although ST adapted faster to the environment and induced earlier hepatocyte TNF-α release.

Formato

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Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652011000200009

Idioma(s)

en

Publicador

Instituto de Medicina Tropical

Fonte

Revista do Instituto de Medicina Tropical de São Paulo v.53 n.2 2011

Palavras-Chave #Listeria monocytogenes #Salmonella Typhimurium #Hepatocytes #Apoptosis #Tumor necrosis factor-alpha
Tipo

journal article