NMR studies of tetrahaem cytochomes from D. desulfuricans and S. frigidimarina

Dissertation Presented to obtain the Ph.D. in Biochemistry

Tetrahaem cytochromes are small soluble proteins with four non-planar haems with bis-histidinyl axial coordination that were first isolated from Desulfovibrio vulgaris in the 1950s and subsequently in several other bacteria. Even though the sequence homology, the amino acid composition and the thermodynamic properties of the several tetrahaem cytochromes may be different; the overall haem core architecture and the general fold of the protein established by X-ray crystallography and nuclear magnetic resonance (NMR) are highly conserved.(...)