Structural and mechanistic studies of iron containing proteins

Dissertação apresentada para obtenção do Grau de Doutor em Bioquímica, ramo de Bioquímica-Física, pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Over the last few decades a large effort has been done in the structural biochemistry field. This effort is based on the study of some proteins, namely metalloproteins, that contain cofactors and/or active sites with metal ions in their constitution. This thesis will focus on different studies performed in metalloproteins that contain non-heme iron centers. An important point is their relation to oxygen and reactive oxygen species. To perform these studies, fast kinetic techniques were used coupled to spectroscopic techniques, such as Electronic Paramagnetic Resonance (EPR), Mössbauer and UVVisible.

Fundação para a Ciência e Tecnologia (SFRH/BD/18905/2004)