Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types


Autoria(s): Malta-Vacas, Joana; Aires, C.; Costa, P.; Conde, A. R.; Ramos, S.; Martins, A. P.; Monteiro, C.; Brito, Miguel
Data(s)

10/12/2013

10/12/2013

01/06/2005

Resumo

Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p , 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.

Identificador

Malta-Vacas J, Aires C, Costa P, Conde AR, Ramos S, Martins AP, Monteiro C, Brito M. Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. J Clin Pathol. 2005 Jun;58(6):621-5.

1472-4146

http://hdl.handle.net/10400.21/2982

Idioma(s)

eng

Publicador

BMJ

Relação

http://jcp.bmj.com/content/58/6/621.long

Direitos

openAccess

Palavras-Chave #DNA, Neoplasm/genetics #Gene dosage #Neoplasm proteins/genetics #Neoplasm proteins/metabolism #Peptide termination factors/genetics #Peptide termination factors/metabolism #RNA, Neoplasm/genetics #Reverse Transcriptase Polymerase Chain Reaction/methods #Stomach neoplasms #Up-regulation
Tipo

article