In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods


Autoria(s): Amador, Paula; Fernandes, Rúben; Duarte, I.; Brito, Luísa; Prudêncio, Cristina
Data(s)

03/02/2014

03/02/2014

2011

25/07/2013

Resumo

The principal aim of this study was to investigate the possibility of transference to Escherichia coli of β-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 β-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX, bla SHV and bla OXY, but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.

Identificador

DOI 10.1007/s11274-010-0635-9

0959-3993

1573-0972

http://hdl.handle.net/10400.22/3601

Idioma(s)

eng

Publicador

Springer

Relação

World Journal of Microbiology and Biotechnology; Vol. 27, Nº 8

http://link.springer.com/article/10.1007%2Fs11274-010-0635-9

Direitos

openAccess

Palavras-Chave #Ready-to-eat (RTE) food #β-lactam-resistant Enterobacteriaceae #bla TEM-1 gene #Transconjugants
Tipo

article