Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents


Autoria(s): Barbosa,Leticia Mascarenhas Pereira; Paiva Neto,Vespasiano Borges de; Dias,Leonardo Lucas Carnevalli; Festucci-Buselli,Reginaldo Alves; Alexandre,Rodrigo Sobreira; Iarema,Lourdes; Finger,Fernando Luis; Otoni,Wagner Campos
Data(s)

01/04/2013

Resumo

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

Formato

text/html

Identificador

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2013000200002

Idioma(s)

en

Publicador

Universidade Federal de Viçosa

Fonte

Revista Ceres v.60 n.2 2013

Palavras-Chave #oxidative stress #cytokinin #lipid peroxidation #micropropagation
Tipo

journal article