Heat resistance of Bacillus spores when adhered to stainless steel and its relationship to spore hydrophobicity


Autoria(s): Simmonds, P. M.; Mossel, B. L.; Intaraphan, T.; Deeth, H. C.
Contribuinte(s)

B. Brannen

Admin Editors

Data(s)

01/01/2003

Resumo

Twenty-one strains of Bacillus (10 B. stearothermophilus, 3 B. cereus, and 8 B. licheniformis strains) were assayed for spore surface hydrophobicity on the basis of three measures: contact angle measurement (CAM), microbial adhesion to hydrocarbons (MATH), and hydrophobic interaction chromatography (HIC). On the basis of the spore surface characteristics obtained from these assays, along with data on the heat resistance of these spores in water, eight strains of Bacillus (three B. stearothermophilus, three B. cereus, and two B. licheniformis strains) either suspended in water or adhering to stainless steel were exposed to sublethal heat treatments at 90 to 110degreesC to determine heat resistance (D-value). Significant increases in heat resistance (ranging from 3 to 400%) were observed for the eight strains adhering to stainless steel. No significant correlation was found between these heat resistance increases and spore surface characteristics as determined by the three hydrophobicity assays. There was a significant positive correlation between the hydrophobicity data obtained by the MATH assay and those obtained by the HIC assay, but these data did not correlate with those obtained by the CAM assay.

Identificador

http://espace.library.uq.edu.au/view/UQ:65829/Deeth_heat_resistance_of_bacillus.pdf

http://espace.library.uq.edu.au/view/UQ:65829

Idioma(s)

eng

Publicador

International Association for Food Protection

Palavras-Chave #Biotechnology & Applied Microbiology #Food Science & Technology #Surface Hydrophobicity #Electrical-properties #Cereus Spores #Adhesion #Milk #Subtilis #Bacteria #Stearothermophilus #Temperature #Attachment #C1 #290104 Other Food Sciences #670107 Grain mill products, starch and starch products (incl. sugar, bakery products)
Tipo

Journal Article