Genomovar diversity amongst Burkholderia cepacia complex isolates from an Australian adult cystic fibrosis unit


Autoria(s): Kidd, T. J.; Bell, S. C.; Coulter, C.
Contribuinte(s)

I. Braveny

Data(s)

01/01/2003

Resumo

In this study, a combination of recA-based PCR assays and 16S rDNA restriction fragment length polymorphism (RFLP) analysis was used to determine the genomovar diversity of clinical Burkholderia cepacia complex isolates. Twenty-eight isolates were prospectively collected from patients attending a large Australian adult cystic fibrosis (CF) unit, 22 isolates were referred from other Australian CF units and a further eight isolates originated from patients without CF. The 28 prospectively collected isolates were distributed amongst the following genomovars: Burkholderia cepacia genomovar I (28.6%), Burkholderia multivorans (21.4%), Burkholderia cepacia genomovar III (39.3%), Burkholderia vietnamiensis (3.6%) and Burkholderia ambifaria (7.1%). The results of this study highlight the usefulness of 16S rDNA RFLP typing for the identification of other Burkholderia spp. and non-fermenting gram-negative bacteria.

Identificador

http://espace.library.uq.edu.au/view/UQ:65751

Idioma(s)

eng

Publicador

Springer-Verlag

Palavras-Chave #Infectious Diseases #Microbiology #Epidemiology #Infection #Identification #Vietnamiensis #Multivorans #Pcr #C1 #321027 Respiratory Diseases #730110 Respiratory system and diseases (incl. asthma)
Tipo

Journal Article