Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues


Autoria(s): Cool, S. M.; Sayer, R. E.; van Heumen, W. R.; Pickles, J. O.; Nurcombe, V.
Contribuinte(s)

P.J. Stoward

Data(s)

01/01/2002

Resumo

Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development.

Identificador

http://espace.library.uq.edu.au/view/UQ:64269

Idioma(s)

eng

Publicador

Kluwer Academic publishers group

Palavras-Chave #Cell Biol #Signal-transduction #Fgf Receptor #Genomic Structure #Skeletal-muscle #Breast-cancer #Tyrosine #Gene #Cells #Mechanisms #Family #C1 #320602 Cell Physiology #1116 Medical Physiology
Tipo

Journal Article